This service allows users to conduct their own fragment analysis (e.g. T-RFLP, ARISA, Microsatellites) on the 3130XL sequencer. It includes the consumables and sequencer maintenance. Depending on the application, labelled primer aliquots, DNA polymerase enzymes, restriction enzymes, purification kits and size markers are available and billed separately.
Sequencing and molecular biology
EMBRC-France offers the necessary tools to carry out sequencing projects and / or genotyping. The platform is organized into two sites: Platform Genomer the Roscoff Biological Station, Platform BIO2MAR the Oceanographic Observatory of Banyuls-sur-mer.
Expertise
- Banks preparations
- Sequencing
- Genotyping
- Handling automation
Associated services (bioinformatics)
- Data preprocessing
- Assembly
- Similarity search
- Annotation
- Bioinformatics data processing
Service Offer
There are several types of access to service: independent access, access with accompaniment or remote service.
Involvement in networks and infrastructure
- Biogenouest
- Bio2Mar
Labeling and certification
Roscoff IBISA certified and ISO 9001
Prestations
Fragment analysis
Prestation :
Analysis by authorised users (trained by facility staff)
Services :
Sites :
Equipment made available
Prestation :
Services :
Sites :
Support for the use and availability of
- Miseq
- Capillary sequencer (3130xl Life technologies)
- Bioanalyzer
- Covaris
- Qubit
- Labchip GXt
- Quantitative PCR (Lightcycler 480-Roche
Equipment made available
Prestation :
Services :
Sites :
- Microplate reader facilities available after receiving training from the facility technical staff.
- Use of the StepOne machine plus qPCR for authorised users (trained by facility technical staff).
- Use of Biomek 3000 for authorised users (trained by facility technical staff)
- ABI310 sequencer for SSCP analysis: authorised users (trained by facility technical staff) can carry out SSCP analysis on the ABI310 sequencer. The service includes formamide, size markers, consumables and sequencer maintenance.
- Sanger 3130 sequencer: authorised users (trained by facility staff) can carry out Sanger sequencing on the 3130XL sequencer.
- Use of the OPTIMA MAX XP ultra-centrifuge with the TL-100 rotor available to authorised users (trained by facility staff)
Purification of PCR product
Prestation :
This service includes purification of the PCR products carried out by the facility’s technical staff and includes the consumables and the purification kit.
Services :
Sites :
Sequencing
Prestation :
Sanger sequencing performed by platform staff
Services :
Sites :
This service includes Sanger sequencing performed by platform staff on purified DNA (e.g. purified PCR products, plasmid DNA). It includes cycle sequencing reagents (BigDye Terminator kit), the purification kit, sequencer and sequencer maintenance consumables. Universal primers are provided; other specific sequencing primers must be provided with the DNA sample by the client. This service can be combined with the purification of PCR product service for non-purified PCR products.
Sequencing
Prestation :
Services :
Sites :
- Banks preparations
- Sequencing with MiSeq (Illumina)