SP5 equipped with a conventional scanner and a resonant scanner ; SP8 confocal microscope using hybrid detector
Microscopy
Electronical Microscopy
Preparation of biological samples for MET observation:
- Fixation, dehydration and inclusion for ultrastructural analysis
- immunocytochemical techniques with labelling to colloidal gold in pre or post coating,
- Cryo-techniques, cryo-substitution, cryo-cuts,
- Negative staining of macromolecules and cellular fractions
- Ultrathin sections and cryo-sections followed contrast
Preparation of biological samples for SEM observation:
- Metallisation and desiccation by bypassing critical point
Optical microscopy (photonics)
- Visualization and localization of fluorescent molecules in confocal microscopy and video microscopy
- Study of the dynamics of migration and subcellular compartments.
- Spectral analysis to autofluorescence
- Multidimensional acquisition field video microscopy to track events on live samples,
- Three-dimensional reconstruction, quantification (fluorescence, counting, pattern recognition, etc.)
- Mosaic
- Processing and Image Analysis (Fiji, Imaris)
Prestations
Scanning Microscopy
Prestation :
Confocal microscopy
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Confocal microscopy
Prestation :
Confocal microscopy observation
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SP5 Leica equipped with a conventional scanner and a resonant scanner
Transmission electron microscopy
Prestation :
Structural and functional observations of cells or tissues in marine model organisms.
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Samples can be prepared upon request (ultrastructural microscopy, immunohistochemistry, negative staining).
Full field microscopy
Prestation :
Full field microscopy
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Fluorescence with photo capture
5D Multidimensional Acquisition
Use of upright epifluorescence microscope
Prestation :
Full-field microscopy
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Use of transmission electron microscope
Prestation :
Electronic microscopy: Structural and/or functional observation of cells or tissues in marine model organisms.
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Preparation and observation of samples.
Use of inverted epifluorescence microscope
Prestation :
Full field microscopy
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